missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Protein Analysis Reagents
  4. Protein Purification
  5. Protein Purification Reagents and Kits
  6. Macherey-Nagel™ Protino Ni-NTA Agarose for His-tag Protein Purification

Macherey-Nagel™ Protino Ni-NTA Agarose for His-tag Protein Purification

Product Code. 11912422
Change view
Click to view available options
Quantity:
25 mL
500 mL
Unit Size:
25mL
500mL
Product Code. Quantity unitSize
11912422 25 mL 25mL
11952192 500 mL 500mL
2 options
This item is not returnable. View return policy

Product Code. 11912422

Brand: Macherey-Nagel™ 745400.25

Please to purchase this item. Need a web account? Register with us today!

This item is not returnable. View return policy

Description

Method of choice for His-tag protein purification with best performance.

  • Enable fast and convenient purification of recombinant polyhistidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).
  • Proteins from any expression system can be purified under native or denaturing conditions.
  • Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions.
  • Consists of the chelating ligand nitrilotriacetic acid (NTA) immobilized on 6% cross-linked agarose beads that are suitable for batch binding, gravity flow, and FPLC™ columns.
  • The resin is precharged with Ni2+ ions and therefore ready to use.
  • Technology: Immobilized metal ion affinity chromatography
  • Batch binding, gravity flow column chromatography, MPLC / FPLC applications
  • Matrix: 6 % beaded agarose (cross-linked), precharged with Ni2+
  • Bead size: 45 to 165 μm

Specifications

Binding Capacity <50 mg/mL settled agarose
Description Aqueous suspension
Content And Storage 4°C to 8°C
Quantity 25 mL
For Use With (Application) Purification of His-tag proteins
Target Protein
Type Agarose
Ligand Type NTA (nitrilotriacetic acid)

Safety and Handling

compliance-icons compliance-icons
Signal Word
  • Warning
 Hazard Category
  • Flammable liquid Category 3
  • Skin sensitiser Category 1
 Hazard Statement
  • H226-Flammable liquid and vapour.
  • H317-May cause an allergic skin reaction.
 Precautionary Statement
  • P210-Keep away from heat, hot surfaces, sparks, open flames and other ignition sources. No smoking.
  • P261-Avoid breathing dust/fume/gas/mist/vapours/spray.
  • P280-Wear protective gloves/protective clothing/eye protection/face protection.
 Supplemental information
  • MIXTURE LIST-Contains : ethanol (diluted < 20 %) (denatured with1%IPA/1%MEK, acc.2016/1867/EU), resin suspension with Ni in
Product Title
Select an issue

By clicking Submit, you acknowledge that you may be contacted by Fisher Scientific in regards to the feedback you have provided in this form. We will not share your information for any other purposes. All contact information provided shall also be maintained in accordance with our Privacy Policy.